Composite

Part:BBa_K3077200:Design

Designed by: Wong Annabelle Tin Lam   Group: iGEM19_Hong_Kong_UCCKE   (2019-10-14)


Expression of lipIAF5-2 under constitutive promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 497
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 497
    Illegal NgoMIV site found at 323
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NA


Source

BBa_K3077202 is from Uncultured Bacterium

References

[1] Meilleur C., Hupe J.F., Juteau P., Shareck F(2009). "Isolation and characterization of a new alkali-thermostable lipase cloned from a metagenomic library."J. Ind. Microbiol. Biotechnol. 36:853-861(2009)
[2] Brault G, Shareck F, Hurtubise Y, Lépine F, Doucet N (2014) Short-Chain Flavor Ester Synthesis in Organic Media by an E. coli Whole-Cell Biocatalyst Expressing a Newly Characterized Heterologous Lipase. PLoS ONE 9(3): e91872. https://doi.org/10.1371/journal.pone.0091872